Date of Award

1996

Document Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

Department

Biology

Abstract

Homeobox genes are known to code for transcription factors regulating developmental programs that are universally represented in animals and plants. Among three $\alpha$-helices and a more flexible fourth helix, helices 3 and 4 recognize specific binding sites of DNA. Amino acid sequences, WFQNRR, are well conserved among both animals and plants. With primers corresponding to these amino acids, putative homeobox genes (Mrx series) are isolated from maize roots through the PCR technique. The translated amino acids are only conserved 17% comparing atypical homeodomains. Based on ectopic expression in Nicotiana tabacum, it is an atypical homeodomain that is expressed in the root epidermis near the root cap at the early embryoid stage. After two or three weeks, regenerated roots possess a drastically changed phenotype.

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