Date of Award

Fall 12-1-2003

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Chemistry

First Advisor

Stephen F. Wolf

Second Advisor

William H. Flurkey

Third Advisor

John M. Allen

Abstract

High performance liquid chromatography has been coupled with online inductively coupled plasma mass spectrometry detection for the determination of iodine speciation in drinking and groundwaters. Using ion exchange chromatography, the method is applied to quantify the concentrations of iodine species in groundwaters. The HPLC-UV detection was used first to examine the separation efficiency of the HPLC method. The limit of quantification was obtained for the HPLC-UV analysis. Direct ICPMS analysis of iodine species has been validated and optimized using an HC03"/C03 2 - aqueous solvent. HPLC was then interfaced to the ICPMS T-shape connection to allow online addition of internal standard. Calibration curve was obtained daily prior to sample analyses. The iodine concentrations in groundwater samples were assayed through the online Time Resolved Analysis. In comparison with the HPLC-UV, the HPLC-ICPMS method is much more sensitive and is the best analytical method for trace iodine speciation analysis in ground water. The method can potentially be extended to applications in clinical and food science areas.

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