Date of Award

2017

Document Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

Department

Biology

Abstract

Hormonal pleiotropy describes a suite of hormonally-linked traits. Such hormonal mediation may facilitate or constrain adaptive evolution of individual traits in the suite. Melanization, regulated in part by hormonal signals, can be tightly linked to non-color traits such as aggression, hormones, and immunity. While most studies examine within-population variation, I studied correlates of melanization, comparing both between- and within-population differences of adult male western fence lizards, Sceloporus occidentalis . I compared multiple high- and low-elevation populations in California, where populations are increasingly darker at higher elevations. I examined variation in 1) agonistic behavior of male lizards in response to staged territorial intrusions (STIs), 2) levels of the understudied peptide hormone, α-MSH, 3) both baseline- and post-captivity stress (1 hr) levels of two steroid hormones (testosterone, corticosterone), 4) mite loads, and 5) wound healing. Analysis of responses to STIs revealed that high-elevation males were more physically aggressive than males in lower-elevation populations. Populations did not differ in mean plasma α-MSH concentrations, but both testosterone and corticosterone levels differed significantly between populations, with higher aggression associated with lower testosterone. I also found darker (high-elevation) populations had higher mean mite loads than the less melanized (low-elevation) populations. Wound healing rate following a standardized wounding procedure, has faster for darker (high-elevation) males compared to lighter (low-elevation) males. Hormonal pleiotropy within the melanocortin system appears to not constrain phenotypic variation in Sceloporus lizards. I found differences in some traits across populations, but no association of individual variation in traits with individual variation in melanization. Physiological mechanisms contributing to within-population differences in melanization may be disassociated from mechanisms contributing to population-level differences. I discuss factors that could favor enhanced melanization at higher elevations. More studies comparing melanization, trait associations, and fitness consequences, both within and across populations, are warranted.

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