Date of Award
1997
Document Type
Dissertation
Degree Name
Doctor of Philosophy (PhD)
Department
Biology
Abstract
The envelope glycoprotein gp52, of Friend spleen focus-forming virus (F-SFFV) is responsible for the disease induced in mice by Friend virus (FV), which is a complex of F-SFFV and Friend murine leukemia virus (F-MuLV). The murine Fv-2 gene controls the susceptibility to F-SFFV infection. Mice resistant at the Fv-2 locus can rarely be infected with the wild-type F-SFFV. The viruses isolated from resistant FV-induced diseased mice have an altered envelope glycoprotein. It has been demonstrated that interaction between gp52 and erythropoietin receptor (EpoR) is required for the disease production in mice. Furthermore, it has been postulated that Fv-2 gene modifies this interaction. EpoR has been shown to have two distinct binding sites: one for erythropoietin and one for gp52. Interaction between EpoR and gp52 takes place on the cell surface and involves the transmembrane domain of EpoR. The first objective of this investigation was to demonstrate that RB virus, a recently cloned variant of F-SFFV could induce disease in both sensitive and resistant mice. This study shows that the cloned RB virus was able to induce disease in both sensitive and resistant mice. The second objective of this study was to determine whether EpoR is involved in Fv-2-mediated resistance. The EpoR from two pairs of mice each congenic at the Fv-2 locus were cloned and sequenced. No common pattern of difference was detected. However, there were some differences in nucleotide sequence between each pair of congenic mice. Whether these differences play any role in the susceptibility to FV remains to be determined.
Recommended Citation
Basu, Subimal, "Characterization Of Congenic FV-2(SS) And FV-2(RR) Mice With Respect To Nucleotide Sequence Of Erythropoietin Receptor And Disease Production By A Variant RB Virus" (1997). All-Inclusive List of Electronic Theses and Dissertations. 1879.
https://scholars.indianastate.edu/etds/1879